Genomics Core Laboratory


Overview of Services

Our mission is to provide UAMS investigators with convenient access to the latest technologies for genomic interrogation using Next Generation Sequencing (NGS), microarray, and ddPCR analysis.

The complete NGS service includes:

  • nucleic acid extractions & QC
  • library preparation & QC
  • sequencing instrumentation setup
  • bioinformatics QC
  • sequence alignment
  • molecular characterization of mutational findings
  • report generation (annotated Excel) 
Illumina Sequencers
HiSeq3000
NextSeq500
MiniSeq
Nucleic Acid extraction
DNA/RNA from cells, solid tissue, FFPE 
cfDNA from plasma, urine
NGS Library preparation
WGS with NEBNext Ultra II
Targeted DNA panels with QIAseq
RNA-seq with TruSeq stranded total RNA Gold
RNA-seq with Takara SMARTer stranded total RNA
QC for Nucleic Acids & NGS Libraries
Fragment Analyzer capillary electrophoresis system
Qubit fluorometeter
Library functional assessment with qPCR 
Droplet Digital PCR (ddPCR)
BioRad QX200 system
Illumina Infinium mircoarray
Methylation EPIC for standard or FFPE DNA
DNA Size Selection
Sage Science PippinHT

  

Leadership

Donald J. Johann, MSc, MD
Scientific Director
DJJohann@uams.edu

Owen W. Stephens, MB(ASCP)
Technical Director
(501) 526-6000 x 24316
StephensOwenW@uams.edu

Location and hours of operation

Hours Location


Monday - Friday 


 8 AM - 5 PM

   
Winthrop P. Rockefeller Cancer Institute

University of Arkansas for Medical Sciences

4301 W. Markham St., slot # 753

Little Rock, AR 72205

 

Links and Resources

  1. UAMS Genomics Shared Resource Website
  2. Illumina coverage calculator

 

Contacts

Name Role Phone Email Location
Owen Stephens
Technical Director
 
(501) 526-6000 x 24316
 
StephensOwenW@uams.edu
 
WPRCI 338
 
Donald Johann
Scientific Director
 

 
DJJohann@uams.edu
 

 
Amanda Metz
Technologist
 
(501) 526-6990 x 8372
 
Ametz@uams.edu
 
WPRCI 338
 
Aisulu Yavas
Technologist
 
(501) 526-6990 x 8372
 
Ayavas@uams.edu
 
WPRCI 338
 

Service list


Search available services: View: by category alphabetically
Nucleic Acid / Library handling (5)
NGS library preparation (7)
NGS Informatics (1)
Microarray (2)
Sequencing (1)
Consulting (1)
Droplet Digital PCR (1)